Development of a Mixture of Dietary Carotenoids as Cancer Chemopreventive Agents: C57BL/6J Mice as a Useful Animal Model for Efficacy Studies with Carotenoids

Introduction ..........................................................................................................163 Design and Formulation of Multicarotenoid Mixture (MCM) ............................166 Preparation of Western-Style Diet with and without Multicarotenoid Mixture (MCM) ....................................................................................................167 Study Design ........................................................................................................169 Tissue Extraction of Carotenoids .........................................................................170 HPLC Analysis of Carotenoids ............................................................................170 Histopathology ......................................................................................................171 Results and Discussion .........................................................................................172 Acknowledgments ................................................................................................174 References ............................................................................................................174