ABSTRACT
GV V $VVD\
INTRODUCTION
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PBL Isolation
+XPDQ 3%/V Z SURWRFRODQGWKHQLV
PBL Exposure
+' RU LWV VLQJO GLOXWHG LQ530,PH WRQ07KHW KU WKHQ SODFHG LQ D SHULRG,PPHGLDWHO\ H[SRVHGWR H[SRVHGFRQWUROV
ODU WDUJHW RI +' LQ WKH HSLGHUPLV 8VLQJ KXPDQ HSLGHUPDO DQGKXPDQSHULSKHUDOEORRGO\PSKRF\WHV3%/VLQFXOWXUH IIHFWV RI +' RQ '1$ DV DVVHVVHG E\ WKH FRPHW VLQJOH FHOO ,QDGGLWLRQZHHYDOXDWHGVXUYLYDODQGJURZWKRI+(.VWKURXJK YLDEOHFHOOQXPEHUPHWDEROLFÁ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ÀFDQW'1$GDPDJHDQGVWLOOVXUYLYHWKHLQVXOW LRFKHPLFDOHIIHFWVDW WKLV ORZHUOHYHORIH[SRVXUHPD\OHDGWR VXUHVDJDLQVWWKHKLJKHULQMXULRXVOHYHOVRI+'GDPDJHVHHQLQ V
PURPOSE
MATERIALS AND METHODS
HUH FROOHFWHG IURP YROXQWHHUV XVLQJ DQ DSSURYHG KXPDQ XVH RODWHGE\EXR\DQWGHQVLW\FHQWULIXJDWLRQ
HDUP PXVWDUG DQDORJ FKORURHWK\O HWK\O VXOÀGH &((6 ZDV GLXPDQGDGGHG WR WKH WXEHV\LHOGLQJÀQDO FRQFHQWUDWLRQVRI
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HEK Cultures
3ULPDU\FXOWXUHV &ORQHWLFV'LY&DPE FPWLVVXHFXOWXUHÁD WKHQ VXEFXOWXUHG DV $OOFXOWXUHVZHUHPD
HEK Exposure
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Comet Assay
%RWKFHOO W\SHVX SURWRFRO6LQJKHWDO VRIWZDUH.LQHWLF,P PRPHQWµ LH SHUFH QHJDWLYHFRQWUROVFH
Viability Assays
$W R ZHOOVZHUHZDVKHGW $0QJP/ZDV IRUWRKU7KHS (PIRUDOD
Growth Curve As
7KH FHOO VXVSHQV EXIIHUHG VDOLQH &HO FRXQWHU &HOO YLDELOL )$&6RUWÁRZF\WRP FDOFXODWHGDVWKHSUR
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say
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RESULTS
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CONCLUSIONS
REFERENCES
