chapter  1
30 Pages

Isolation and Purification of DNA

Deoxyribonucleic acid (DNA) isolation and purification are essential techniques for molecular biology studies. The quality and integrity of the isolated DNA directly affect the results of all subsequent scientific research.1-4 A number of methods have been developed for extraction and purification of DNA from different tissue types and organisms. We present several well-developed protocols here for the isolation of DNA from bacteria, λ phages, animals, and plants, and for the purification of DNA fragments from agarose gels.