chapter  8
20 Pages

Immobilized synthetic dyes in affinity

Affinity chromatography (Clonis, 1990, 2000; Labrou and Clonis, 1994; Garg et al., 1996; Finette et al., 1997; Keller et al., 2001; Lowe, 2001) is the most powerful technique used in protein purification, especially in cases where the quality criteria imposed on the final product are stringent. In principle, affinity chromatography exploits natural biorecognition phenomena for the formation of specific reversible complexes between a ligand, immobilized on an insoluble porous support packed in a column, and the complementary ligand-binding sites on the biomolecule to be isolated.