chapter  14
46 Pages

Capillary electrokinetic chromatography

High performance liquid chromatography and capillary zone electrophoresis are two high resolution separation processes based on the principle of differential migration and widely used for the analysis of non-volatile substances. HPLC employs pressure driven flow of the liquid mobile phase through a column packed with the stationary phase and the sample components are separated due to differences in their partitioning between the two phases. Following the trend towards miniaturization HPLC is increasingly carried out with packed fused silica capillary columns (µ-HPLC), mainly to facilitate applications involving its tandem use with the mass spectrometer.