ABSTRACT

Manufacturer: Genentech, Inc., 1 DNA Way, South San Francisco, CA, https://www.gene.com/ (U.S.)

Marketing: Genentech, Inc., 1 DNA Way, South San Francisco, CA, https://www.gene.com/ (U.S.)

Manufacturing

Nutropin Depot contains the same somatropin human recombinant growth hormone as Nutropin and NutropinAq, presented as a different formulation. The recombinant hormone is produced in E. coli using recombinant DNA technology. A bacterial signal sequence is added in front of the hormone nucleotide sequence in order to avoid addition of a methionine residue at the protein N-terminus during production in the bacterium. The expressed protein is transported into the E. coli periplasmic space, during which the additional N-terminal sequence is removed, resulting in a functional recombinant protein.