ABSTRACT
The biochemical induction assay (BIA) involves the monitoring of the lysogenic induction of a lambda-lac z fusion phage by using Escherichia coli (strain BR 513). The lac z gene of this organism, which is responsible for the production of ( -glycosidase, is fused to the promoter of phage lambda, and the expression of this gene causes what is known as an “'SOS” response and results in the production of -glycosidase which is measured colorimetrically.
