ABSTRACT
Advances in sensor technology often involve development of new ways of detecting a particular analyte or creation of new sensing methodologies that can be applied to a variety of sensors. Here we describe an example of each of two modes of analytical sensor research, both focused on novel ways to apply fluorescence spectroscopy to the detection of cellular analytes. The first section describes developments in optical sensors for detection of cellular nitric oxide. Two biosensors and one chemical sensor have been developed that are highly selective and reversible and have fast response times. These sensors have been applied to extracellular and intracellular measurements of macrophage produced nitric oxide.
