ABSTRACT
ABSTRACT: In this study, the technology process of DECB preparation was optimized, which was expected to improve the quality of the product, protect its biological activities and shorten its production cycle for the achievement of an industrial production of DECB. Under a sterile condition, the blood from calves at ages of 2-6-months was sterilized and filtrated by pressing calves; The filtrate was adjusted to different solutions at different pH values, which were ultra-filtrated to remove the proteins, adjusted their pH to the neutral, and then concentrated, sterilized and packaged; the breathing activity (QO2) of it was measured with a Warburg micro breathing pressure meter, and the Stimulation Index (SI) was calculated; According to the pyrogenic reaction test described in Pharmacopoeia of Peoples republic of China, the pyrogenic reaction of samples was observed. The results showed that when the filtrate was adjusted to one at an acidic pH of 3 or an alkaline pH of 9, the breathing activity was strongest and the pyrogenic reaction was lowest. The results indicated that DECB prepared by the improved technology in this study should have a higher biological activity with less pyrogenic reaction, and the preparation method of DECB should be simple, easily operated and feasible.
