ABSTRACT
ABSTRACT: In this study, we developed and optimized an efficient enzyme-assisted protein depletion procedure for polysaccharides from Angelica and Astragalus. The Percentage of the Removed Protein (PRP) served as an evaluation index to compare the deproteinization effects of the Sevag method, TCA method and enzyme-assisted method. Processing parameters for enzyme-assisted deproteinization conditions were optimized using four factors at three levels of orthogonal design based on single-factor experiments. The results showed that the optimal conditions were as follows: papain concentration of 0.3%, deproteinization time of 120 min, enzyme action temperature of 55°C and pH of 5.0. Under these conditions, the PRP was 75.4 ± 0.10%, which is basically in accord with the model predicted value of 75.0%.
