ABSTRACT

ABSTRACT: A Dynabead-labeled sandwich immunoassay had been developed using a Giant Magnetoimpedance (GMI) biosensor for the detection of C-Reactive Protein (CRP). The biosensor involving the sensing elements of Cr/Cu/NiFe/Cu/NiFe was fabricated by Micro Electro-Mechanical System (MEMS) technology. The immune reaction of biomarkers CRP was accomplished on a separated Au film substrate surface with a self-assembled layer. The fundamental principle for detection of CRP based on GMI biosensor was that Dynabeads were employed as magnetic labels of CRP, and CRP can be monitored by detecting the fringe field of Dynabeads using magnetic sensing elements. We observed that the GMI ratio were significantly enhanced due to the presence of CRP combined with Dynabeads. The rise decreased as the CRP concentration increased. A lower detectable concentration of 10 ng/ml was achieved in present work. The GMI-based biosensor provides a new method to rapid and sensitive detection CRP, which has a large potential for bio-application.