ABSTRACT
ABSTRACT: An Ionic Liquid (IL)-containing buer system was first applied in the conversion of isoeugenol to vanillin by Bacillus fusiformis CGMCC1347. High substrate solubility was achieved to enhance the eciency of the reaction. Nine ILs were selected as co-solvents to assist catalytic reactions in this biotransformation process. 1-ethyl-3-methylimidazolium methylsulfate [C_2mim][MetSO_4] was the suitable ionic liquid to be tested for this biotransformation. The optimal biotransformation conditions were as follows: Ionic liquid 40 μL, isoeugenol 400 μL for 10 mL reaction liquid in 50-mL flask, phosphate buer (pH 7.0) 50 mM, 37oC, 180 r/min for 60 h. The Maximum vanillin concentration reached 1.38 g/L.
