ABSTRACT

ABSTRACT: A study on relationship between Sulfate-Reducing Bacteria (SRBs) in groundwater under the coal mine and coal samples in Permian formation taken from the Huainan Panbei coal mine was done. The water samples from borehole were detected with 16SrRNA gene sequencing and systematically compared. A SRB strain of Desulfovibrio genus was detected out. A GC-MS test matched 21 macromolecule organic compounds by methanol extraction of coal samples. From SRB-carbon source adaptability comparison test, it is found that butyleret hydroxytoluen and 2-hexyl-1-Decanol were good carbon sources for SRBs’ anaerobic metabolism, while Naphthalene,2,6-dimethyl was not eectively degradaed as the carbon source. The conclusions suggested carbon sources needed by sulfate reduction in groundwater in coal fields could come from surrounding coal seams themselves.