ABSTRACT
Jin-ting He Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China
Le Yan People’s Hospital of Jilin Province, Changchun, China
Jiao-qi Wang & Yang Cui Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China
Zong-Shu Li Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China People’s Hospital of Jilin Province, Changchun, China
ABSTRACT: Ischemic stroke occurs when the blood supply to the brain is obstructed, and it is one of the most common worldwide causes of health problems, disability and death. We investigated the expression of Hes1 of PC12 cells in an Oxygen Glucose Deprivation (OGD) model. In this study, we used nerve growth factor to stimulate PC12 cells and converted them into neuron-like cells, then the OGD of PC12 cells was used to establish a cerebral hypoxia-ischemia model in vitro. Flow cytometry analysis, Western blot, and real-time PCR assays were performed. The results showed that the expression levels of Hes1 mRNA and protein were significantly increased after 3 h of OGD treatment, Our results suggested that OGD can up-regulate the mRNA and protein expression levels of Hes1 cerebral ischemic injury may activate the Notch signaling pathway.
