ABSTRACT
ABSTRACT: To assess the role of Smad7, which mediate the activation of ActA/Smads pathway against ischemia neural injury. The model of PC12 cells with Oxygen Glucose Deprivation(OGD) was built to simulate the process of ischemia neural injury in vitro, Smad7 gene was silenced by using RNA-interfering technology, apoptosis of PC12 cells from ischemia injury was analyzed with DAPI, the expression of p300 and procaspase-3 gene was observed by real-time PCR. Smad7-siRNA can inhibit the expression of Smad7 and reduce the apoptotic rate of PC12 cells caused by ischemia injury, whereas Smad7-siRNA can promote the expression of procaspase-3 protein and downstream gene of signal protein transcription activating factor: p300. Targetsilencing Smad7 gene can enhance the signal transduction pathway of ActA/Smads and reduce the sensitivity of PC12 cell to OGD injury.
