ABSTRACT
At present, many studies show that oxalate not only plays a role in regulating physiological processes such as plant cell Ca2+ concentration, pH, and so on but also plays an important function in plant adaptation to environmental stress (Weir et al. 2006). Dou et al. found that most of Mn in the leaves of Mn hyperaccumulator Phytolacca acinosa exist in the
form of oxalate (Dou et al. 2008). Therefore, oxalate plays an important role in passivating heavy metals and enhancing plant resistance. Oxalate can chelate with toxic metal ion and release their toxic content. It is a response factor of environmental stress in plants (Mazen 2004). As a result, the content determination of oxalate in the sample has also become an important method to research action mechanism of oxalate in the body. Currently, the methods of oxalate content determination mainly include titration, fluorescence analysis, high-performance liquid chromatography (HPLC) (Daxboeck et al. 2003), and so forth. But content determination of oxalate in hyperaccumulator by using ion chromatography is rarely reported. This paper studies the content determination of oxalate in hyperaccumulator, establishing related methods to determine oxalate by ion chromatography.
