chapter  2
66 Pages

Liquid Chromatography/Mass Spectrometry in Forensic Toxicology

Procedures (QQQ, qTOF, and qIT) ........................ 109 2.7 Perspectives and Future Trends.......................................................... 110 References ...................................................................................................... 111

Toxicology may be generally defined as a scientific search for a causal relationship between exposure to a given compound and observed biological effect. Analytical toxicology provides all the technically refined tools indispensable for detection of toxic compounds or — in other words — for the assessment of the exposure. These tools, as well as the whole analytical strategy, may be different in various toxicological disciplines. In forensic toxicology, only those methods may be successfully applied which combine efficient separation of the relevant compound from the biological matrix with the most specific detection. LC/MS fulfils the requirements of forensic toxicological analysis due to high selectivity, possibility of detection of active metabolites, and efficient screening in unclear death cases. For this reason, also, due to gradual introduction of low-cost LC/MS instruments, this technique is now finding an increasingly important place in forensic toxicological laboratories.