ABSTRACT
High performance liquid chromatography (HPLC) takes many different forms and is a primary method of analysis in many types of laboratories. HPLC separates mixtures of compounds into their individual components by means of an interaction of the compound, a liquid mobile phase, and an inert stationary phase. The interactions between the mobile phase and stationary phase are diverse and dependent on the types of compounds undergoing separation and the extent to which separation is required. The output from the data analysis system is a chromatogram. An example of this is shown in Figure 2.1, where the small peak at the beginning is the peak for the unrestrained solute and shows the point at which the injection has been detected (change in pressure of the system). The x-axis represents the time and the y-axis represents the detector response.
