ABSTRACT

A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products a

chapter 1|8 pages

An introduction to PCR

chapter 2|14 pages

Understanding PCR

chapter 3|42 pages

Reagents and instrumentation

chapter 4|22 pages

Optimization of PCR

chapter 7|48 pages

PCR mutagenesis

chapter 8|24 pages

Analysis of gene expression

chapter 9|24 pages

Real-time RT-PCR

chapter 10|24 pages

Cloning genes by PCR

chapter 11|26 pages

Genome analysis