ABSTRACT

Specific microbiological methods are used in environmental engineering systems for: (1) isolation, cultivation, identification, and quantification of pure cultures; (2) selection of strains and construction of recombinant microbial strains; (3) selection and quantification of enrichment cultures; (4) identification and quantification of microorganisms in environmental samples without cultivation; (5) extraction, cloning, enrichment, and identification of microbial genes and their products in environmental engineering systems. A tutorial with solutions and a quiz bank are added to this chapter.