ABSTRACT

The application of fluorescent methods in histochemistry and cytochemistry allows us to determine the presence of certain medicinal compounds (phenols, terpenes, neurotransmitter compounds, and others) in secretory structures containing drugs. It occurs after staining with reagents that induce fluorescence of the -fluorescent or weakly fluorescent substances. It is possible to avoid lengthy biochemical procedures by using a small amount of pharmaceutical material stained with various fluorescent dyes in microscopic analysis. This approach is also useful for the determination of reactive oxygen species accumulated in phytomaterials under stress.