ABSTRACT
This chapter describes the method in detail and focuses on the analysis of the first-order reaction. It also describes how to determine the rate constant of the first-order reaction. The enzyme and substrate are mixed well by the mixing chamber, and travel the flow tube. Enzyme and substrate are mixed by the first mixer, then the reactants are mixed with quencher by the second mixer. The zero-order reaction is the one that the rate is independent of the concentration of reactant. Most of the commercially available stopped-flow apparatus has its own software to fit the data for the first-order reaction and higher-order of reactions, and show the rate constant instantaneously. For the detection of the radioactivity, the reaction mixture obtained by the rapid mixing was further mixed with the denaturant to stop the reactions. Then the radioactivity of the reactants was measured.
