ABSTRACT
According to the “central dogma of molecular biology,” DNA is transcribed into RNA and then translated into protein. Current methods of DNA sequencing (known as next-generation approaches) generate immense amounts of sequence data. In some cases, entire genomes are not necessary. For example, in population genetics studies detection of nucleotide variants in a genome is sufficient. RNA-Seq allows for examination of the entire collection of RNA transcripts in different kinds of cells, tissues, or organisms. This can also be used to generate sequences for phylogenetic analysis.
