ABSTRACT
Glycan enrichment can be performed using various ways, i.e. using a diverse range of physical or chemical interactions, their combination and finally using biospecific/affinity-based recognitions using lectins. Application of hydrazide-modified materials is another popular choice for glycan enrichment. Hydrophilic interaction-based method for glycan enrichment is also a popular pre-concentration method involving use of a polar stationary phase and a nonpolar mobile phase for separation. Silica Nanoparticles are frequently applied for glycan enrichment, are mechanically and chemically stable over a wide pH, a feature important for column packing material. A simplified glycan enrichment procedure within a pipette tip for example was based on application of 20 nm Gold Nanoparticles attached to the polymer backbone to enhance surface area. Molecularly imprinted polymers (MIPs) can be effectively applied in a selective capture and release of glycoproteins, since the analyte molecule is imprinted and after formation of a thin layer of a polymer around the template, the analyte is removed from the MIP.
