ABSTRACT
Wherever possible, tables are arranged according to classes of chemical compounds. This was not always possible when different chemical compounds were chromatographed under the same experimental conditions. The reader is referred to the compound index for specific compounds that may appear in different tables. <target id="page_136" target-type="page">136</target>Soap Tlc of Peptides
Layer
LI
L2
L3
L4
L5
L6
Solvent
SI
SI
SI
SI
SI
SI
Detection
D1
D1
D1
D1
D1
D1
Technique
T1
T1
T1
T1
T1
T1
Peptide
RF × 100
Gly-Gly
96
76
60
88
68
44
Gly-Ala
96
68
55
85
62
39
Gly-Ser
96
81
71
93
75
53
Gly-Ile
83
22
14
38
22
9
Gly-Leu
81
20
12
32
20
8
Gly-Pro
96
ES
51
75
56
28
Gly-Tyr
90
44
33
59
44
22
Gly-Phe
72
18
12
29
18
7
Ala-Ala
95
60
48
74
58
36
Ala-Gly
96
74
60
87
67
43
Ala-Ser
96
77
70
92
71
50
Ala-Val
90
37
25
52
37
20
Ala-Ile
77
21
14
33
21
9
Ala-Pro
92
48 a
43
67
49
24
Ala-Tyr
90
40
30
54
41
20
Ala-His
96
16
6
38
12
2
Asp-Gly
96
ND
92
95
78
58
Asp-Ala
96
ND
78
88
68
49
Phe-Gly
76
19
13
32
19
8
Phe-Ala
75
17
12
26
15
7
Arg-Gly
96
6
3
21
5
2
Arg-Asp
96
11
5
29
9
3
Ile-Gly
90
30
24
47
35
14
Leu-Leu
55
6
5
9
5
3
Leu-Val
71
13
11
20
12
7
Leu-Tyr
72
16
14
24
15
8
Gly-Gly-Gly
96
75
56
83
66
42
Gly-Gly-Ala
96
64
49
78
59
38
Gly-Ala-Gly
96
67
49
78
59
38
Gly-Ala-Ala
96
60
46
74
56
36
Ala-Ala-Ala
96
61
51
74
57
38
Gly-Gly-Phe
76
24
16
29
17
7
Gly-Leu-Tyr
63
13
12
17
12
6
Leu-Gly-Phe
47
5
4
9
5
2
Gly-Gly-Gly-Gly
96
75
56
83
64
39
Ala-Ala-Ala-Ala
96
63
55
75
59
41
Leu-Trp-Met-Arg
49
0
0
0
0
0
Leu-Trp-Met-Arg-Phe
11
0
0
0
0
0
Note: ES, elongated spot. ND, not determined.Tailing
Layer: L1 = SiO2. L2 = SiO2 + 4% DBS (triethanolamine dodecylbenzenesulfonate). L3 = SiO2 + 4% Na-DSS (sodium dioctylsulfosuccinate). L4 = SiO2 + 1% H-DBS (dodecylbenzenesulfonic acid). L5 = SiO2 + 2% H-DBS. L6 = SiO2 + 4% H-DBS. Layers are prepared by mixing 20 g of silanized silica gel 60 HF (C2) (Merck) in 50 mℓ of 95% ethanol with a known concentration of detergent. Detergent concentration refers to the ethanolic solution in which the silica gel is suspended. 137Solvent: S1 = Water-methanol-acetic acid, 64.3:30:5.7 v/v. Detection: D1 = The wet layers are sprayed with 1% ninhydrin in pyridine-glacial acetic acid, 5:1 and the layers are then heated at 100°C for 5 min. Technique: T1 = Temperature, 25°C; migration distance, 11 cm.