ABSTRACT
Most of the elements of any control system look inwards at one another—in the immune response, most lymphocytes interact with each other rather than with the external environment. Thus, the role of idiotype-bearing and anti-idiotypic T cells in the regulation of the immune response has been the subject of numerous investigations. Work in a laboratory has been directed to understanding the immune response to the ferredoxin (Fd) molecule. The finding that FdB2 id+ T cells and their respective id– counterparts controlled the magnitude of immune responses to Fd in mice of various haplotypes, was strongly suggestive of network interaction. B16G was used to identify T cell hybridomas capable of secreting molecules with which it reacted. In order to determine the nature of the Fd11 TsF material, Fd11 culture supernatants were affinity purified over B16G immunoadsorbent columns, eluted using either pH 4.3 or pH 1.0 buffers, run on sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and silver stained.
