ABSTRACT
Plant research often involves growing new plants in a controlled environment. In plant cell culture, plant tissues and organs are grown in vitro on artificial media, under aseptic and controlled environment. The technique depends mainly on the concept of totipotentiality of plant cells which refers to the ability of a single cell to express the full genome by cell division. Plant growth regulators (PGR's) play an essential role in determining the development pathway of plant cells and tissues in culture medium. As an emerging technology, the plant tissue culture has a great impact on both agriculture and industry, through providing plants needed to meet the ever increasing world demand. It has made significant contributions to the advancement of agricultural sciences in recent times and today they constitute an indispensable tool in modern agriculture. Aseptic technique is absolutely necessary for the successful establishment and maintenance of plant cell, tissue and organ cultures.
