ABSTRACT
Transgenic and gene targeting technologies have led to marked advances in the understanding of the molecular basis of electrophysiology in the mouse (1,2). The ability to manipulate the genetic makeup of organisms by targeting selected genes has provided a novel means of investigating the mechanisms underlying ion channel function. As an increased number of research labs have used genetically altered mice in their studies, it has become clear that this technique offers novel opportunities but also raises questions specific to each field. Some concerns arise as a result of the genetic manipulation, which can (1) affect development and lead to compensatory changes in the organism or (2) affect multiple organs and lead to secondary effects. Other concerns derive from the use of the mouse as the model, whose physiology may differ radically from that of larger mammals.
