This chapter discusses the so-far achieved success in the field of aggregation of luminophores. Initially, the application of the aggregation of luminophores in biological fields is discussed. In biological areas, luminophores are mainly used as probes for the sensing of biogenic species, cell imaging, integrated with other function for diagnosis and/or therapy. In view of this, this section is split into two smaller sections as biomolecular sensing and biological imaging. Next, the application of the aggregation of luminophores in chemical sensing is illustrated. The aggregation phenomenon utilized for ion detection, pH sensing, gas detection, explosive detection, fingerprint visualization, and chiral recognition will be sequentially introduced in this section. Then, the application of aggregation of luminophores in optoelectronic systems such as organic Light-Emitting Diodes (OLEDs), photovoltaics, liquid Crystals (LCs) and organic Field-Effect Transistors (OFETs) are discussed. Finally, the application of luminophores in the drug delivery field is described. The design of drug delivery approaches leveraging supramolecular chemistry (i.e., “chemistry beyond the molecule”) has earned significant interest in recent years toward improving therapeutics. The design opportunities furnished by supramolecular chemistry will play a vital role in the future of the drug delivery field. Finally, conclusions and future outlook are presented and discussed.