ABSTRACT
New methods for the analysis of edible oils, fats, and cellular lipids have recently been developed, presented at scientific meetings, and published in peer-reviewed journals. These methods apply to biological and food matrices, edible oils and fats, as well as cellular fats of pathogenic bacteria and spores, and will cover many research applications in lipidomics, food analysis, food safety, food security, and counter-terrorism. This text offers the lipid analyst essential analytical tools in the fields of chromatography, mass spectrometry, spectroscopy, magnetic resonance, and chemometrics. It also serves as a reference for recent developments in the rapidly evolving field of lipid methodologies.
OVERVIEW. Techniques and Applications in Lipid Analysis. MASS SPECTRAL TECHNIQUES/LIPIDOMICS. An Overview of Modern Mass Spectrometry Methods in the Toolbox of Lipid Chemists and Biochemists. Global Cellular Lipidome Analyses by Shotgun Lipidomics Using Multidimensional Mass Spectrometry. LC/MS and Chiral Separation. LC/MS and Lipid Oxidation. Structural Analysis of Unsaturated Fatty Acid Methyl Ester Isomers with Acetonitrile Covalent Adduct Chemical Ionization. CHROMATOGRAPHIC TECHNIQUES. Recent Advances in Silver-Ion HPLC Utilizing Acetonitrile in Hexane as Solvent: Temperature Effects on Lipid Elution Orders/Resolution. Analysis of trans-18:1 Fatty Acids by Silver Ion HPLC. High-Performance Size-Exclusion Chromatography for Lipid Analysis in Organic Media. Lipid Separations Using Packed-Column Supercritical Fluid Chromatography. TLC-FID with Special Reference to Marine Lipids and Other High-Molecular-Weight Organic Compounds. Fast GC for Cellular FAME Analysis of Bacteria. VIBRATIONAL SPECTROSCOPIC TECHNIQUES. Use of Cellular Fatty Acids to Identify Food-Borne Pathogens by Infrared Spectroscopy and Capillary Gas Chromatography. Application of FT-NIR for Rapid Determination of the Trans Fatty Acid Composition in Fats and Oils. Infrared Spectroscopy and Partial Least Square Calibration in the Simultaneous Quantification of Isolated trans and Conjugated Linoleic Acids. Investigation of Protein-Lipid Interactions by Vibrational Spectroscopy. APPLICATIONS. Fat Replacers: An Overview. Phospholipids: Structures and Physicochemical Activities. Waxes and Sterols: Structures and Chemistry.
