ABSTRACT
Chromatography separation is a quasi-equilibrium process. The characteristics are largely determined by the properties of the solid support being used and those of the sorption layer formed by it in the column. This chapter considers the effect of the basic parameters of the solid support (or, to be more exact, the sorbent) on the efficiency of the chromatographic column. It interprets the experimental results with regard to the height equivalent to one theoretical plate versus particle diameter and other parameters. The adsorptional properties of a solid support substantially affect the efficiency of chromatographic separation. The qualitative effect of the adsorptional properties of a solid support shows up distinctly enough when comparing the efficiency of an adsorbent based on the initial adsorptionally active support with that of an adsorbent based on a modified solid support, the adsorptional activity of which is suppressed by introducing polar and surface-active compounds into stationary-liquid-phase.
