ABSTRACT

Plant regeneration by tissue culture is now an essential and fundamental procedure for biotechnology and plant breeding, and is used commercially for the asexual propagation of many horticultural and agricultural plants. Since then protocols and procedures for somatic embryogenesis have been developed for several species and these are used both for the regeneration of plants and as a research model to study early morphogenetic and regulatory events in plant embryogenesis. In addition to somatic embryogenesis, regeneration can be accomplished through other developmental pathways. All the plants derived by somatic embryogenesis from a particular tissue culture are genetically alike. Consequently, micropropagation through somatic embryogenesis is an efficient method of producing large numbers of identical elite or transgenic plants. The regeneration of plants from tissue explants by organogenesis is extensively used commercially for the micropropagation of economically important plants.