ABSTRACT
The prime purpose of a tissue culture laboratory is to enable the processing and culture of plant material in a sterile environment. To facilitate this purpose, certain basic facilities are required. These usually include the following: a media preparation and sterilization area; a sterile transfer area; and environmentally controlled incubators or a culture room. Most plant tissue culture facilities use a laminar flow cabinet (or hood) to provide an aseptic area for transfer work. These units are available commercially in different sizes and can be placed where required in the culture laboratory. In laboratories where large numbers of cultures are routinely processed or large pieces of equipment are required, a useful arrangement would be a dust-free sterile transfer room. In general, plant tissue cultures are particularly sensitive to environmental conditions and should be grown under conditions where air circulation, temperature, humidity, light quality and photoperiod are well controlled.
