ABSTRACT

Whilst the preceding five chapters have dealt with the basic principles and methods of nucleic acid electrophoresis, in this second part of the book the focus is on the techniques and application of different electrophoretic separations. A consistent format will be used for each chapter. The approach will be to outline the principles of a method and then to focus on published research articles to explain what preparatory steps were taken, how the electrophoretic separation was analyzed and what information was obtained. Recipes and lists of chemicals are not the concern of this introductory text, but for each method suitable sources will be referenced in the Protocols box at the head of each chapter. This will enable a worker with little experience to establish a new technique or refine an existing application once they have become familiar with the fundamental principles described in this book.