ABSTRACT
The goal of fluorescence photomicroscopy is to reproduce on to a permanent medium the image seen by the observer through the microscope as accurately as possible. Historically, film was the first medium employed to capture images through the microscope, and today still remains the preferred means of preserving specimen information. Fluorescence photomicroscopy is usually a race between integrating enough intensity on the storage medium before the specimen is damaged. Colour slide film is the most reliable medium for photomicroscopy. Most black and white film is developed by the user in a darkroom. This allows substantially more flexibility to the user in terms of producing negatives and images of high resolution and contrast. The quality, contrast and grain of the film’s emulsion is controlled by how the film is handled, the time of development, agitation and temperature.
