ABSTRACT

This introduction presents an overview of the key concepts discussed in the subsequent chapters of this book. The book explains the principles of the methods used currently in the analysis of lipids to assist those wishing to understand or plan experiments in the area. Analysis is assumed to involve recognition, isolation and quantitative assay of a compound or group of compounds. The book summarizes much of the general information pertinent to preparing an appropriate protocol for individual compounds or groups of compounds. It opens with a brief account of the biological significance of the group of lipids under consideration. This biological context helps in assessment of the types of analytical questions that may need to be answered. All quantitative analytical methods should be supported by good calibration data, preferably calibration curves covering the range of analyte concentrations being reported.