ABSTRACT
For the study of mitosis from the division of the generative nucleus in germinating pollen grains, the methods involve, germination of the pollen tube, and treatment for observation of the chromosomes. For haematoxylin staining, the more prolonged schedule as followed for plants is also applicable in the case of animals. The Newton's crystal violet staining schedule can also be applied to animal materials. In all animal materials, acetic acid-methanol (1:3) is a more effective fixative than acetic acid-ethanol mixture. This chapter provides more effective methods on mammalian chromosomes. For the study of these chromosomes, the salivary glands of different Dipteran larvae are the most suitable material, although they also occur in the cells of malphigian tubules, fat bodies, ovarian nurse cells and gut epithella. Lamp brush chromosomes are very elastic and may be pulled to many times their length without injury.
