ABSTRACT

Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles.

Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.

chapter 1|27 pages

Basic principles of gene transfer

chapter 2|29 pages

Chemical and physical transfection

chapter 3|13 pages

Cell-medicated gene transfer

chapter 4|50 pages

Gene transfer using viral vectors

chapter 5|34 pages

Genetic manipulation of animals

chapter 6|33 pages

Gene targeting in animal cells

chapter 7|34 pages

Control of transgene expression

chapter 8|15 pages

Transgene behavior

chapter 10|18 pages

Applications of gene transfer in animals