ABSTRACT
Analysis of PCR products is critical in optimizing PCR conditions to yield reliable and accurate results, and in interpreting the levels of products generated, for example, in a diagnostic context.
The first part of this chapter considers how to analyze PCR-amplified DNA fragments in the most appropriate way for different experimental strategies. In general it is important to ensure that you develop robust and reproducible PCR conditions, particularly if they are to become part of a routine high-throughput screening procedure. Approaches for detection and analysis of PCR products and for verification of product identity, including direct sequencing strategies, are considered. In the second part, procedures for real-time detection and quantitation of a specific product are covered. The final section deals with in vitro expression of PCR products to yield protein products.
