ABSTRACT
Genome walking is indispensable to extensive structural analysis of large gene segments. A number of genome walking methods are available to the researcher-including iPCR,1,2 cassette ligationanchored PCR,3 vectorette PCR,4 panhandle PCR,5,6 Alu-PCR,7 thermal a symmetric interlaced PCR (TAIL-PCR),8 and EPTS/LM-PCR9 (see also the review by Eric KaWai Hui and Szecheng J. Lo in Chapter 30, and Chapter 31 on iPCR by Jorge A. GarcÉs et al.). However, the usefulness of these techniques has been hampered by a combination of (1) limited sensitivity/specificity, (2) a short walk range, (3) a requirement for restriction enzymes and advantageous restriction maps, (4) a requirement for efficient ligation, (5) the necessity of molecular cloning, and (6) low throughputs or low rates of success.
