ABSTRACT
In a ‘typical’ metabolic problem, rats weighing a total of 1kg were given a single dose of a 14Clabelled drug at 20mg/kg. Liquid scintillation counting showed that 0-48 hour urine (80g) contained 70% (14mg-equivalents) of the dose. Reverse-phase high-performance liquid chromatography (HPLC) with radiochemical detection (Figure 1.1) suggested that the urine contained at least 15 components, of which four accounted for 71% (10mg-equivalents) of the dose recovered in the 0-48 hour urine sample. After being freeze-dried, the urine was found to contain 6g of solid.
