ABSTRACT

A key stage of converting the primary genetic information from the stable DNA code into protein is to translate the messenger RNA (mRNA) into a polypeptide. This process is carried out by the ribosome. The ribosome can only bind to messenger RNA, at sites to the 5’ of the AUG start codon. Confusion often arises over the location of the promoter, the ribosome binding site (RBS), and the transcript start site, as these are often all marked on schematics showing the features of a gene in its genomic location (Table 1).