ABSTRACT
In Asia, most water buffaloes are stall-fed, and most farmers have only a few animals, minimizing exposure to wild canids. Viable N. caninum was isolated from buffalo brains. Brains from 6 asymptomatic culled male buffaloes from Sao Paulo, Brazil were used to attempt isolation of N. caninum using cell culture and bioassay. In a heterogeneous population of buffaloes from various sources in Pakistan aborting buffaloes had a higher seroprevalence versus non-aborting buffaloes. In Asia, most water buffaloes are stall-fed, and most farmers have only a few animals, minimizing exposure to wild canids. For in vitro cultivation, 10 g of brain of each of the 6 buffaloes were digested in trypsin, washed, and homogenates seeded onto bovine monocytes. Transplacental transmission of N. caninum has been induced in buffaloes by IV inoculation of dams with 108 or 5 × 108 tachyzoites.
