ABSTRACT

In vitro bioassays are any qualitative or quantitative analysis that is performed in sterile laboratory environment to measure the activity profile of a drug on a live tissue cell or in a living organism. The three essential components of in vitro bioassays are stimulus like drug candidates; substrate like tissues, cells, biochemicals, and so on; and response of the substrate to various doses of stimulus. Standardized and well-established in vitro techniques are designed for the experimental assessment of efficacies of new anticancer agents and these assays allow reduction in the number of promising agents for further clinical testing. Cell culture techniques employ number of key elements and features that are universally applied, although there can be diverse ways in practicing these procedures. The adherent monolayer is dislodged from the tissue culture flasks when the culture has occupied the complete surface of the flask, using proteolytic enzymes such as trypsin to achieve a single cell suspension to maintain healthy cell lines.