ABSTRACT
This chapter discusses various protein microarray platforms, with special emphasis on cell-free expression based microarrays and various applications of cell-free expression microarrays. The DNA microarrays, also commonly referred to as the DNA chip or biochip, are collection of microscopic DNA probes that are attached on a solid surface. There are three types of protein microarrays include analytical microarrays, functional microarrays and reverse phase protein microarrays. Protein in situ array is a technique which allows the one step rapid generation of protein arrays directly from DNA templates by using cell- free protein expression and simultaneous immobilizations at a surface in situ. The HaloTag can be genetically fused to any protein of interest, and the Halo ligands are a variety of organic molecules that enable irreversible binding to the HaloTag protein. Cell-free synthesis based microarray systems have demonstrated their potential in biomarker discovery and autoantibody profiling as well as applications related to the pharmaceutical industry.
