ABSTRACT
Nonviral approaches has been found to be an excellent and safe alternative gene transfer vehicles to other popular viral vectors due to having significant favorable properties such as lack of immunogenicity, low toxicity, and potential for tissue specificity and targeted drug delivery. Non-viral vectors-based gene therapy using cationic lipids or polymers, gold Nanoparticles, magnetic nanoparticles, quantum dots, silica nanoparticles, fullerenes, carbon nanotubes was found to have promising safe delivery potential as compared to viral gene delivery. Magnetic nanoparticle-based gene transfection has been shown to be effective nonviral nanodevice in both viral vectors and with non-viral gene delivery. Chitosan-DNA-FAP-B nanoparticles were found to be novel non-viral vectors for specific gene delivery to the lung epithelial cells. The potential non-viral gene delivery was also performed with polymeric nanoparticles composed of poly and DNA that can be formulated to be stable in the presence of serum proteins and have high gene delivery without toxicity to human primary cells.
