ABSTRACT

Immunodiagonostic techniques are used in the identification of plant pathogenic bacteria in the laboratory or directly under field condition to identify the pathogen. These are generally used in the identification of plant pathogenic bacteria from the imported seed material or nursery plant material. When a bacteria or virus infects an animal or human being, some particles penetrate and start multiplying in the infected cells. Killer T lymphocytes cause chemical damage to the membranes of the infected cells, thus interrupting virus replication. Immunodiagnostic techniques are helpful in detection of phytobacterial pathogens of plant quarantine importance. Preparation of antibodies through antigens and their uses are essential part of immunodiagnostic technique. Effective immunoglobulin content of different antiserum samples collected during different immunization period and its affinity towards antigen Xanthomonas corylina is determined by using immunofluroscence technique. Immunological Electron Microscopy technique is particularly useful when low bacterial concentration prevents direct observation.