ABSTRACT
To control the bacterial infection, antibacterial compounds are required. The antibacterial compound can be present in certain plants or can be isolated from certain microorganisms. The absence of bacterial endophytes is indicative of probable presence of antibacterial compound in the test plant and the absence of either Gram-negative or Gram-positive bacteria or both is indicative that the antibacterial compound is active against what type of bacteria. For assessment of antibacterial properties of solvent extract, bacterial lawn is prepared by adding bacterial suspension to nutrient agar medium and poured in Petri plates. Identification of antibacterial molecules can be done by using gas chromatography mass spectroscopy (GC.MS) by using Quattro micro GC-MS unit. Centrifuge the sample at 10,000 rpm for 4 minutes at 18°C for purification. Transfer the supernatant to vials for GC-MS analysis.
