ABSTRACT
The antibacterial compound can be present in certain plants or can be isolated from certain microorganisms. Testing of these antibacterial compounds against plant pathogenic bacteria and their identification at molecular level is important. The leaf imprint method is used to numerate bacterial population on the leaf surface of test plants. For numeration of bacterial endophytes in the leaves of test plants, wash the leaves of the respective plants in tap water to remove the dirt. The absence of bacterial endophytes is indicative of probable presence of antibacterial compound in the test plant and the absence of either Gram-negative or Gram-positive bacteria or both is indicative that the antibacterial compound is active against what type of bacteria. Formation of inhibition zone indicate the presence of antibacterial molecules in the plant extract and that the plant part possesses antibacterial properties.
