ABSTRACT
Radiotherapy or radiation therapy is one of the most effective therapies for cancer. Different doses of radiation are needed to kill different types of cancer cells. The aim of this study was to investigate the application of different doses of X-ray irradiation on noncancerous (CHEK-1 cells) and cancerous human esophageal cells (TE-8 cells). CHEK-1 cells and TE-8 cells were cultured using RPMI medium. Different doses of X-ray irradiation (0–8Gy) were applied. The clonogenic assay was performed to assess the survival of both cells. Plating efficiency (PE) and surviving fraction (SF) were calculated. SF between the control and irradiated groups was statistically significantly different (P < 0.05). SF between the noncancerous and cancerous cells was not statistically significantly different (P > 0.05), except for 2Gy X-ray irradiation (P < 0.05). X-ray irradiation starting with a dose of 2Gy can decrease the survival rate of both noncancerous and cancerous cells. Therefore, 2Gy can be used as an irradiation dose for further study.
